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Antibodies
Anti-N6-methyladenosine (m6A) antibody
Anti-N6-methyladenosine (m6A) antibody from Abcam
Be the first to write a review!
Citations:
(123)
Supplier Page
Supplier Page from
Abcam for
Anti-N6-methyladenosine (m6A) antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-N6-methyladenosine (m6A) antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab151230
Quantity
100 UG
Conjugate/Tag
Unconjugated
Isotype
IgG
Host
Rabbit
Antibody Type
Primary
Clonality
Polyclonal
Concentration
1 mg/ml
Applications
Nucleotide Array
Reactivity
Drosophila melanogaster, Human
Target
N6-methyladenosine (m6A)
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (123)
(1)
N6-methyladenosine-regulated ADIRF impairs lung adenocarcinoma metastasis and serves as a potential prognostic biomarker
Cancer Biology & Therapy
December 31, 2023
Yin Teng, Xiaohan Zhao, Yang Xi, Ninghua Fu
purified using DNase (Sigma) and fragmented into ~ 100 nucleotides. m6A primary antibody (10 μg,
Abcam
,
ab151230
) linked to
Magna ChIP protein/G magnetic beads
was co-
immunoprecipitated
with the fragmented mRNA for
(2)
A1BG-AS1 promotes adriamycin resistance of breast cancer by recruiting IGF2BP2 to upregulate ABCB1 in an m6A-dependent manner
Scientific Reports
November 25, 2023
Jian Wang, Jie Xu, Jie Zheng
10 mM Tris–HCl, pH 7.5, 0.1% NP-40) and incubated with magnetic beads coated with IgG and anti-m6A (
ab151230
,
Abcam
, USA) antibodies for 1 h in order for binding. Next, the purified mRNAs and the complex was added
(3)
YTHDF1 mitigates acute kidney injury via safeguarding m6A-methylated mRNAs in stress granules of renal tubules
Redox Biology
November 1, 2023
Wenwen Yang, Mingchao Zhang, Jiacheng Li, Shuang Qu, Fenglian Zhou, Minghui Liu, Limin Li, Zhihong Liu, Ke Zen
and
anti-YTHDF3
(
25537-1-AP
,
1:100
) were from
Proteintech
; anti-
G3BP1
(
ab56574
,
1:200
) and
anti-m6A
(
ab151230
,
1:100
) were from
Abcam
. Secondary antibodies were all from
Invitrogen
(
A-21206
,
A-21203
,
1:1000
). All
(4)
Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m(6) A RNA methylation in ocular melanoma
Cancer communications (London, England)
November 1, 2023
Zhuang A, Gu X, Ge T, Wang S, Ge S, Chai P, Jia R, Fan X.
was randomly fragmented using RNA fragmentation buffer and subjected to IP with an anti‐m6A antibody (
ab151230
,
Abcam
) and PGM magnetic beads in IP buffer supplemented with RNase inhibitor. The
m6A
‐containing RNA
(5)
YTHDF2 promotes gallbladder cancer progression and gemcitabine resistance via m6A‐dependent DAPK3 degradation
Cancer Science
November 1, 2023
Xuesong Bai, Jiemin Chen, Wenqin Zhang, Shengnan Zhou, Liangbo Dong, Jianhao Huang, Xiaodong He
total of 100 μL precleaned Protein G Dynabeads (
10003D
;
ThermoFisher
) was mixed with 5 μg anti‐m6A Ab (
ab151230
;
Abcam
) or IgG isotype control Ab (#
3900S
;
CST
) overnight at 4°C with rotation. The total RNA mixed
(6)
IGF2BP2 acts as a m6A modification regulator in laryngeal squamous cell carcinoma through facilitating CDK6 mRNA stabilization
Cell Death Discovery
October 10, 2023
Xiaojun Tang, Qinglai Tang, Shisheng Li, Mengmeng Li, Tao Yang
cells (Novocyte, Agilent, USA). Methylated m6A
RNA immunoprecipitation
(Me-RIP) Anti-m6A antibody (
ab151230
,
Abcam
, Cambridge, MA, USA) was employed to perform the Me-RIP as previously described [39].
qRT-PCR
(7)
KIAA1429/VIRMA promotes breast cancer progression by m6 A-dependent cytosolic HAS2 stabilization
EMBO reports
October 9, 2023
Na Li, Zhouting Zhu, Yufei Deng, Rachel Tang, Hui Hui, Yuqi Kang, Tariq M Rana
fragmented mRNA was used
for IP
and incubated with 10 μl of 1 μg/μl rabbit anti‐m6A polyclonal Ab (
ab151230
,
Abcam
) in IP‐binding buffer (10 mM Tris–HCl, 150 mM NaCl, 0.1% NP‐40, pH 7.4) for 2 h at 4°C. Protein
(8)
HNRNPC promotes collagen fiber alignment and immune evasion in breast cancer via activation of the VIRMA-mediated TFAP2A/DDR1 axis
Mol Med
August 1, 2023
Bin Lian, Shuxun Yan, Jiayi Li, Zhengyang Bai, Jinping Li
Isolation Systems (61,006, ThermoFisher, USA). For experimental grouping, anti-m6A antibody (
1:500
,
ab151230
,
Abcam
, Cambridge, UK) or anti-IgG antibody (
ab109489
,
1:100
,
Abcam
, Cambridge, UK) were added to IP
(9)
Deubiquitinase USP9X stabilizes RNA m(6)A demethylase ALKBH5 and promotes acute myeloid leukemia cell survival
The Journal of biological chemistry
August 1, 2023
Wang P, Wang J, Yao S, Cui M, Cheng Y, Liu W, Gao Z, Hu J, Zhang J, Zhang H.
no.: sc-8017), K48-linkage specific polyubiquitin (
CST
; catalog no.:
8081
), m6A (
Abcam
; catalog no.:
ab151230
), FLAG (Sigma; catalog no.:
F2922
), HA (
Sigma
; catalog no.:
B9183
), GFP (
Proteintech
; catalog no.:
66002-1-Ig
),
(10)
Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m(6)A-dependent recognition of Nrf2 mRNA mediated by SND1
Journal of experimental & clinical cancer research : CR
May 19, 2023
Zheng J, Zhang Q, Zhao Z, Qiu Y, Zhou Y, Wu Z, Jiang C, Wang X, Jiang X.
Biodyne Nylon Transfer
Membranes
(Pall) and cross-linked for 30 s by UVP (1.2 kJ). The anti-m6A antibody (
ab151230
,
Abcam
) was applied to determine the global m6A level.
Methylated RNA immunoprecipitation
(MeRIP)
assay
(11)
A role for N6-methyldeoxyadenosine in C. elegans mitochondrial genome regulation
bioRxiv
May 17, 2023
Lantana K. Grub, James P. Held, Tyler J. Hansen, Samantha H. Schaffner, Marleigh R. Canter, Evi M. Malagise, Maulik R. Patel
with 5% milk. After blocking, the
membrane
was incubated with the primary antibody, anti-6mA (
Abcam
,
ab151230
), at
1:1000
in TBST with 5% milk overnight at 4 C. The
membrane
was then washed 3 times with TBST for
(12)
RNA demethylase ALKBH5 promotes colorectal cancer progression by posttranscriptional activation of RAB5A in an m6A-YTHDF2-dependent manner
Clinical and translational medicine
May 1, 2023
Shen D, Lin J, Xie Y, Zhuang Z, Xu G, Peng S, Tang G, Bai L, Zhu M, Zhang Y,
et al
.
dot‐blot
assays
as introduced by previous publication. 28 The primary antibody used was anti‐m6A (
Abcam
,
ab151230
). 2.10
RNA sequencing
,
methylated RNA immunoprecipitation
and m6A
sequencing
(MeRIP‐seq), and data
(13)
Upregulation of UBR1 m6A Methylation by METTL14 Inhibits Autophagy in Spinal Cord Injury
eNeuro
April 24, 2023
Changsheng Wang, Xitian Zhu, Rongsheng Chen, Xiaobo Zhang, Nancheng Lian
purified by PolyATtract mRNA Isolation Systems (A-Z5300, A&D. Technology Corporation). Anti-m6A (
1:500
,
ab151230
,
Abcam
) or anti-IgG (
1:100
, Abcam) antibodies were added to the
immunoprecipitation
buffer (20 mm Tris,
(14)
Intestinal transit amplifying cells require METTL3 for growth factor signaling, KRAS expression, and cell survival
bioRxiv : the preprint server for biology
April 21, 2023
Danan CH, Naughton KE, Hayer KE, Vellappan S, McMillan EA, Zhou Y, Matsuda R, Nettleford SK, Katada K, Parham LR,
et al
.
m6A-enriched RNA was
immunoprecipitated
a second time using 2 µg of a second anti-m6A antibody (
Abcam
ab151230
). Final cDNA Libraries were prepared from twice
immunoprecipitated
RNA and fragmented input total RNA
(15)
Nuclear m6A reader YTHDC1 promotes muscle stem cell activation/proliferation by regulating mRNA splicing and nuclear export
eLife
March 9, 2023
Yulong Qiao, Qiang Sun, Xiaona Chen, Liangqiang He, Di Wang, Ruibao Su, Yuanchao Xue, Hao Sun, Huating Wang
Technologies) was washed three times with 1 ml IP buffer and incubated with 2 μg m6A antibody (
Abcam
,
ab151230
) at RT for 30 min. The conjugated Dynabeads-m6A-antibody was washed in 1 ml IP buffer three times and
(16)
METTL3 regulates breast cancer-associated alternative splicing switches
Oncogene
March 1, 2023
Achour C, Bhattarai DP, Groza P, Román ÁC, Aguilo F.
buffer (250 mM Tris pH 7.4, 500 mM NaCl, 0.25% NP-40) and incubated with 10 µg of m6A antibody (
Abcam
,
ab151230
) in the presence of RNase
inhibitors
, for 3 h at 4 °C. 30 µl of prewashed
Surebeads
Protein A magnetic beads
(17)
Evaluation of N 6-methyldeoxyadenosine antibody-based genomic profiling in eukaryotes
Genome Research
March 1, 2023
Brian M. Debo, Benjamin J. Mallory, Andrew B. Stergachis
111–RRID:AB_2619891), referred hereafter as “SS2”;Rabbit polyclonal anti-N6-methyladenosine antibody (
Abcam
ab151230
–RRID:AB_2753144), referred hereafter as “
AB1
”;
Anti-N6-methyladenosine (m6A
) antibody, rabbit monoclonal
(18)
Identification of miR-30c-5p as a tumor suppressor by targeting the m(6) A reader HNRNPA2B1 in ovarian cancer
Cancer medicine
February 1, 2023
Wu Q, Li G, Gong L, Cai J, Chen L, Xu X, Liu X, Zhao J, Zeng Y, Gao R,
et al
.
with 10%
goat
serum. The sections were incubated with primary antibodies against m6A (
1:200
,
Abcam
,
ab151230
) at 4°C overnight and cultured with the biotin‐conjugated IgG the next day. The sections were then stained
(19)
PCIF1-mediated deposition of 5'-cap N(6),2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection
Proceedings of the National Academy of Sciences of the United States of America
January 31, 2023
Wang L, Wang S, Wu L, Li W, Bray W, Clark AE, Gonzalez GM, Wang Y, Carlin AF, Rana TM.
material, and the remaining 90% was subjected to
immunoprecipitation
with anti-m6A antibody (
Abcam
,
ab151230
).
Immunoprecipitated
RNA was eluted with RLT buffer (QIAGEN, 160051456), purified by ethanol precipitation,
(20)
New insights on the interaction between m6A modification and non-coding RNA in cervical squamous cell carcinoma
World Journal of Surgical Oncology
January 30, 2023
Guqun Shen, Fen Li, Yan Wang, Yongmei Huang, Gulibiya Aizezi, Jinrui Yuan, Cailing Ma, Chen Lin
total RNA was extracted from C33A cells by TRIzol (Invitrogen, CA, USA). One microgram of m6A antibody (
ab151230
;
Abcam
) was coupled to the mixture of Protein A beads (Thermo Fisher Scientific, Waltham, MA). Later,
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Anti-N6-methyladenosine (m6A) antibody from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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